Netflix's The Roast of Tom Brady, part of the Netflix Is A Joke Fest, was held at the Kia Forum in Los Angeles.
The event, which was broadcast live and unedited, saw many famous faces in attendance, including Ben Affleck and Kim Kardashian.
Invited on stage by comedian Kevin Hart, Kardashian began her roast amidst initial cheers that were quickly drowned out by boos.
The jeering was so intense that Kardashian had to pause mid-sentence, looking stunned at the audience.
It was only after Hart's intervention that the crowd calmed down.
The footage showed NFL legend Tom Brady also taking to the stage, delivering a joke about Kardashian's ex, Kanye West, and their four children, leaving viewers stunned.
Brady joked, "Not because of this but because her kids are at home with their dad."
"Not because of this, but because her kids are home with their dad."
After briefly opening her mouth, the ex-wife of Kanye West swiftly resumed a tight smile, locking eyes with Brady.
Kim was seen shaking her head, and blinking slowly, while the remainder of the audience burst into laughter.
The edited version includes a disclaimer stating it was 'edited from a previous livestream'.
In this version, Kardashian walks on stage, high-fives Hart, and delivers her jokes without any noticeable jeering from the crowd.
The incident was discovered by the online community, which quickly discussed it heatedly on social networks
One person said: Poor Kim
A second wrote: She’s got a new career as a comedian now.
While a third commented: They cut out the best part? Egregious.
Another added: It wasn’t edited when we watched it! I think she handled it great
Someone else said: I noticed that when I watched it. Also the laughing at her jokes sounded like such canned laughter.
Kim Kardashian wore a silver Maison Margiela corset with a cardigan at the Met Gala.
Kim drew attention in a silver bodysuit from the Maison Margiela brand.
The upper part was like a corset, tightly constricting the mother-of-four's 22-inch bust, while her "huge" hips and hourglass figure made her waist appear even smaller.
However, Kim Kardashian, 44, sparked controversy with her waist-hugging outfit, leaving fans worried about her health.